S1-07#

  • Datum izdelave: 2026-05-07

  • Koda seminarja: S1-07

KO POTRES MINE, SE ZAČNEJO NADURE#

Medtem ko ste bili na kosilu, je nenadoma prišlo do potresa. Ko prispete nazaj v laboratorij, na tleh opazite kup neoznačenih epic. Ti pripadajo vašemu sodelavcu, ki je zaradi potresa utrpel zlom noge. Ker mora obiskati urgenco, vas prosi, če mu lahko pomagate ugotoviti kateri vzorci so v posameznih epicah in naredite nekaj dodatnih analiz. Po sikvenciraju vzorcev dobite dve nukleotidni zaporedji:

Zaporedje, ki kodira protein 1:

ATGGCCGACGCAGCCATCCATGGCCACGAGCACGACCGGAGGGGGTTCTTCACCCGCTGGTTCATGTCGA
CGAACCACAAGGACATCGGCGTTCTCTATCTCTTCACCGGGGGCCTCGTCGGGCTGATCTCGGTGGCCTT
CACCGTCTACATGCGGATGGAGCTCATGGCGCCGGGCGTGCAGTTCATGTGCGCCGAACATCTAGAATCG
GGCCTCGTGAAGGGCTTCTTCCAGTCGCTCTGGCCCTCGGCGGTGGAAAACTGCACCCCGAACGGCCATC
TGTGGAACGTCATGATCACCGGCCACGGGATCCTGATGATGTTCTTCGTGGTCATTCCCGCGCTCTTCGG
CGGCTTCGGCAACTATTTCATGCCGCTGCACATCGGCGCGCCGGACATGGCCTTCCCGCGGATGAACAAC
CTCTCCTACTGGCTCTATGTCGCGGGCACCTCGCTCGCCGTCGCCTCGCTCTTCGCGCCGGGCGGCAACG
GCCAGCTGGGCTCTGGCATCGGCTGGGTGCTCTATCCGCCGCTCTCCACCTCCGAATCGGGCTATTCCAC
CGACCTCGCGATCTTCGCGGTGCACCTGTCGGGCGCCTCCTCGATCCTCGGCGCGATCAACATGATCACG
ACCTTCCTGAACATGCGCGCACCCGGCATGACCATGCACAAGGTGCCGCTCTTCGCCTGGTCGATCTTCG
TCACCGCCTGGCTGATCCTGCTGGCGCTGCCCGTGCTCGCCGGCGCCATTACCATGCTGCTGACCGACCG
GAACTTCGGCACCACCTTCTTCCAGCCCTCGGGCGGCGGCGACCCGGTGCTCTACCAGCACATCCTGTGG
TTCTTCGGCCACCCGGAGGTCTACATCATCGTGCTGCCGGCCTTCGGCATCGTCAGCCACGTCATCGCGA
CCTTCGCCAAAAAGCCGATCTTCGGCTATTTGCCGATGGTCTATGCGATGGTGGCGATCGGCGTGCTGGG
CTTCGTGGTCTGGGCCCACCACATGTATACAGCCGGCCTGAGCCTCACCCAGCAGAGCTACTTTATGATG
GCGACCATGGTGATCGCAGTGCCCACCGGCATCAAGATCTTCTCCTGGATCGCGACCATGTGGGGCGGCT
CGATCGAGCTCAAGACGCCGATGCTCTGGGCGCTCGGGTTCCTCTTCCTCTTCACCGTGGGCGGCGTCAC
CGGCATCGTGCTGAGCCAGGCGAGCGTCGACCGCTATTATCACGACACCTACTATGTCGTGGCGCACTTC
CATTATGTGATGAGCCTCGGCGCGGTCTTCGGCATCTTCGCAGGGATCTACTTCTGGATCGGCAAGATGT
CGGGCCGGCAATATCCGGAATGGGCCGGGAAGCTGCATTTCTGGATGATGTTCGTGGGCGCGAACCTCAC
CTTCTTCCCGCAGCACTTCCTCGGCCGCCAGGGCATGCCGCGGCGCTACATCGACTATCCCGAGGCCTTC
GCCACCTGGAACTTCGTCTCGTCGCTGGGCGCCTTCCTGTCCTTCGCCTCGTTCCTCTTCTTCCTCGGCG
TGATCTTCTACACGCTGACGCGCGGGGCGCGGGTGACGGCGAACAACTACTGGAACGAACATGCCGACAC
GCTGGAGTGGACGCTGACCTCTCCGCCGCCGGAGCATACGTTCGAGCAGCTTCCCAAGCGGGAAGACTGG
GAACGCGCGCCCGCCCACTGA

Zaporedje, ki kodira protein 2:

ATGGGGCTGGCCTGGGGACTCGGTGTCCTGCTCCTGTTGCATGCCTGCGGCTCCAACCGCATTCCAGAGT
CTGGGGGAGACAACAGTGTGTTTGACATCTTTGAACTCACCGGAGCTGCCCGCAAGCGGTCTGGGCGCCG
ACTGGTGAAGGGCCCTGACCCTTCTAGCCCAGCTTTCCGCATCGAGGATGCCAACCTGATCCCCCCTGTG
CCTGACAAGAAGTTCCAAGACCTAGTGGATGCTGTGCGGGCGGAGAAAGGTTTCCTCCTCCTGGCCTCCC
TGAGGCAAATGAAGAAGACCCGGGGTACCCTGCTGGCTGTGGAGCGGAAAGACCACTCTGGCCAGGTCTT
CAGCGTGATCTCCAATGGCAAGGCGGGCACCCTGGACCTGAGCCTGACCGTGCAGGGGAAGCAGCATGTG
GTGTCGGTGGAAGAAGCACTCCTGGCGACTGGCCAGTGGAAGAGCATCACCCTGTTTGTGCAGGAGGACA
GGGCCCAGCTGTACATCGACTGTGAGAAGATGGAGAATGCGGAGCTGGATGTCCCCATCCAGAGCATCTT
CACCAGGGACCTGGCCAGCATCGCCAGGCTCCGCATTGCCAAAGGAGGTGTCAACGACAATTTCCAGGGG
GTGCTGCAGAATGTAAGGTTTGTCTTTGGAACCACACCAGAAGACATCCTCAGGAACAAAGGCTGCTCCA
GCTCTACCAGTGTCTTTGTCACCCTTGACAACAACGTGGTGAATGGGTCCAGCCCTGCCATCCGCACCGA
CTACATTGGCCACAAGACAAAGGACCTGCAAGCCATCTGTGGCATCTCATGTGACGAGCTGTCCAGCATG
GTCCTGGAGCTCAGGGGTCTACGCACCATCGTGACCACGCTGCAGGACAGTATCCGCAAAGTGACCGAAG
AGAACAAAGAGCTGGCCAACGAGCTGAGGAGGCCCCCACTCTGCTACCACAACGGAGTGCAGTACAGGAC
TGGCGACGAGTGGACGGTGGACAGCTGCACTGAGTGTCGCTGCCAGAACTCAGTTACCATCTGCAAAAAA
GTGTCCTGTCCCATCATGCCCTGCTCCAATGCCACAGTTCCGGATGGAGAATGCTGCCCACGGTGCTGGC
CCAGCGACTCTGCAGACGATGGCTGGTCCCCGTGGTCTGAGTGGACCTCTTGCTCTGTGACCTGTGGCAA
TGGAATCCAGCAGCGTGGCCGCTCCTGCGACAGCCTCAACAACAGATGCGAGGGCTCCTCTGTGCAGACG
CGGACCTGCCACATCCAGGAGTGTGACAAGAGATTTAAACAGGATGGCGGCTGGAGCCACTGGTCCCCAT
GGTCATCTTGCTCCGTAACATGTGGAGACGGTGTGATCACAAGGATCCGGCTCTGCAACTCCCCCAGCCC
CCAGATGAATGGGAAGCCATGTGAGGGCAAAGCCCGGGAGACCAAAGCCTGCCAGAAAGACTCCTGCCCC
ATCAATGGAGGCTGGGGACCTTGGTCACCATGGGACATCTGTTCTGTCACCTGTGGAGGAGGGGTACAGA
AACGTAGCCGGCTCTGCAACAACCCCAAACCCCAGTTTGGAGGCAAGGACTGCGTTGGTGATGTGACAGA
AAACCAGATCTGCAACAAGCAGGACTGTCCCATTGACGGATGCCTGTCCAATCCCTGCTTTGCTGGTGTC
CAGTGTACCAGCTACCCTGATGGCAGCTGGAAGTGTGGTGCCTGTCCCCCAGGCTATAGTGGAGATGGAG
TCGAGTGCAAAGACGTTGATGAGTGCAAAGAAGTCCCTGATGCCTGCTTCAACCACAATGGAGAGCACAG
GTGTGAGAACACAGACCCCGGCTACAACTGCCTGCCCTGCCCACCGCGCTTCACTGGCTCGCAGCCCTTT
GGCCGGGGCGTGGAACATGCCACCGCCAACAAGCAGGTATGCAAGCCCCGAAACCCCTGCACAGACGGGA
CACACGACTGCAACAAGAACGCCAAGTGCAACTACCTGGGCCACTACAGCGACCCCATGTACCGCTGCGA
GTGCAAGCCTGGCTACGCCGGCAACGGCATCATCTGCGGGGAGGACACAGACCTGGACGGCTGGCCCAAT
GAGGACCTGCTGTGCGTGGCCAACGCAACTTACCACTGCAGAAAGGATAATTGCCCCAACCTTCCCAACT
CAGGGCAGGAAGACTATGACAAGGATGGAATCGGCGATGCCTGCGATGATGACGATGACAATGATAAGAT
TCCAGATGACAGGGACAACTGTCCATTCCATTACAACCCAGCCCAGTACGACTATGACAGAGATGACGTG
GGAGACCGCTGTGACAACTGCCCCTACAACCACAACCCAGACCAGGCTGACACAGATAACAATGGGGAAG
GAGACGCCTGTGCAGCTGACATTGATGGGGACAGTATCCTCAATGAACGGGACAACTGCCAGTATGTCTA
CAATGTGGACCAGAAAGACACTGACATGGACGGGGTTGGTGATCAGTGTGACAACTGCCCCCTGGAACAC
AATCCAGACCAGCTCGACTCTGACTCGGACCGCATTGGAGACACCTGTGACAACAATCAGGATATTGATG
AAGACGGCCACCAGAACAATCTGGACAACTGTCCCTACGTGCCCAACGCCAACCAGGCTGACCATGACAA
GGATGGCAAAGGCGATGCCTGTGACCATGATGACGACAATGATGGCATTCCTGATGACCGGGACAACTGC
AGGCTGGTGCCCAATCCTGACCAGAAGGACTCTGATGGTGATGGTCGAGGTGATGCTTGCAAAGATGATT
TTGACCAGGACAAGGTGCCAGACATTGATGACATCTGTCCCGAAAATGTTGATATCAGTGAGACTGATTT
CCGCCGATTCCAGATGATTCCTCTAGATCCCAAAGGGACATCCCAGAATGACCCTAACTGGGTTGTACGC
CATCAGGGTAAAGAACTCGTCCAGACTGTCAACTGTGACCCTGGACTTGCTGTAGGTTATGACGAATTTA
ACGCCGTGGACTTCAGTGGCACCTTCTTCATCAACACCGAGAGGGATGACGACTATGCCGGCTTTGTGTT
TGGCTACCAGTCCAGCAGCCGCTTCTATGTTGTGATGTGGAAGCAAGTCACTCAGTCCTACTGGGACACC
AACCCCACGAGGGCTCAGGGGTACTCTGGACTTTCCGTGAAGGTTGTAAACTCCACCACGGGGCCTGGCG
AGCACCTGCGGAATGCCCTGTGGCACACAGGAAACACCTCTGGCCAGGTGCGCACACTGTGGCATGACCC
TCGTCACATTGGCTGGAAAGATTTCACTGCCTACAGATGGCATCTGAGCCACAGGCCAAAGACAGGTTTC
ATCAGAGTGGTAATGTATGAAGGGAAGAAAATCATGGCTGACTCAGGACCCATCTATGACAAAACCTATG
CTGGTGGGAGGCTAGGCTTGTTCGTCTTCTCTCAAGAAATGGTGTTCTTCTCCGACCTGAAATATGAATG
CAGAGACTCCTAA

ORGANIZMI V FASTA FORMATU#

  1. Katerima organizmoma pripadata zaporedji?

  2. Kje v celici najdemo protein 1 in kakšno maso ima (Da)?

  3. Kako dolg je signalni peptid zaporedja 2?

  4. Kakšne posttranslacijske modifikacije so prisotne pri proteinu 2?

  5. Poišči homolog proteina 1 v organizmu 2.

  • Kakšen je max score prvega zadetka?

  • Zapiši Uniprot kodo homologa.

  • Zapiši na katerih mestih v zapisu proteina je transmembranska regija 6.

  1. Koliko znanstvenih člankov, ki v naslovu vsebujejo znanstveno ime organizma 2, je bilo objavljenih med letoma 2020 in 2022 (vključno s tema dvema letoma)?

OD REZULTATOV DO NOVIH POVEZAV#

Vaš sodelavec je še vedno na bolniški. Ker ste pomagali že pri identifikaciji vzorcev, vas je vodja raziskovalne skupine zadolžil še za izvedbo nadaljnjih analiz. Preiskujete človeški spondin 1. Spondin 1 je adhezijski protein, ki usmerja in pomaga pri rasti aksonov (pazi! Iskati je potrebno Spondin-1).

  1. Napiši aminokislinski ostanek, ki se pojavlja v vezavnem mestu spondina 1.

  • Kateri ion se veže na to mesto?

  1. Je ta protein podoben kateremu od zgornjih proteinov? Če ja, koliko mest je identičnih?

  2. V katerem ekspresijskem sistemu je bila izražena struktura spondina 1 z resolucijo 2.00 Å?

  3. Kakšna je izoelektrična točka spondina 1?