# S1-07 - **Datum izdelave**: 2026-05-07 - **Koda seminarja**: S1-07 ## **KO POTRES MINE, SE ZAČNEJO NADURE** Medtem ko ste bili na kosilu, je nenadoma prišlo do potresa. Ko prispete nazaj v laboratorij, na tleh opazite kup neoznačenih epic. Ti pripadajo vašemu sodelavcu, ki je zaradi potresa utrpel zlom noge. Ker mora obiskati urgenco, vas prosi, če mu lahko pomagate ugotoviti kateri vzorci so v posameznih epicah in naredite nekaj dodatnih analiz. Po sikvenciraju vzorcev dobite dve nukleotidni zaporedji: Zaporedje, ki kodira protein 1: ``` ATGGCCGACGCAGCCATCCATGGCCACGAGCACGACCGGAGGGGGTTCTTCACCCGCTGGTTCATGTCGA CGAACCACAAGGACATCGGCGTTCTCTATCTCTTCACCGGGGGCCTCGTCGGGCTGATCTCGGTGGCCTT CACCGTCTACATGCGGATGGAGCTCATGGCGCCGGGCGTGCAGTTCATGTGCGCCGAACATCTAGAATCG GGCCTCGTGAAGGGCTTCTTCCAGTCGCTCTGGCCCTCGGCGGTGGAAAACTGCACCCCGAACGGCCATC TGTGGAACGTCATGATCACCGGCCACGGGATCCTGATGATGTTCTTCGTGGTCATTCCCGCGCTCTTCGG CGGCTTCGGCAACTATTTCATGCCGCTGCACATCGGCGCGCCGGACATGGCCTTCCCGCGGATGAACAAC CTCTCCTACTGGCTCTATGTCGCGGGCACCTCGCTCGCCGTCGCCTCGCTCTTCGCGCCGGGCGGCAACG GCCAGCTGGGCTCTGGCATCGGCTGGGTGCTCTATCCGCCGCTCTCCACCTCCGAATCGGGCTATTCCAC CGACCTCGCGATCTTCGCGGTGCACCTGTCGGGCGCCTCCTCGATCCTCGGCGCGATCAACATGATCACG ACCTTCCTGAACATGCGCGCACCCGGCATGACCATGCACAAGGTGCCGCTCTTCGCCTGGTCGATCTTCG TCACCGCCTGGCTGATCCTGCTGGCGCTGCCCGTGCTCGCCGGCGCCATTACCATGCTGCTGACCGACCG GAACTTCGGCACCACCTTCTTCCAGCCCTCGGGCGGCGGCGACCCGGTGCTCTACCAGCACATCCTGTGG TTCTTCGGCCACCCGGAGGTCTACATCATCGTGCTGCCGGCCTTCGGCATCGTCAGCCACGTCATCGCGA CCTTCGCCAAAAAGCCGATCTTCGGCTATTTGCCGATGGTCTATGCGATGGTGGCGATCGGCGTGCTGGG CTTCGTGGTCTGGGCCCACCACATGTATACAGCCGGCCTGAGCCTCACCCAGCAGAGCTACTTTATGATG GCGACCATGGTGATCGCAGTGCCCACCGGCATCAAGATCTTCTCCTGGATCGCGACCATGTGGGGCGGCT CGATCGAGCTCAAGACGCCGATGCTCTGGGCGCTCGGGTTCCTCTTCCTCTTCACCGTGGGCGGCGTCAC CGGCATCGTGCTGAGCCAGGCGAGCGTCGACCGCTATTATCACGACACCTACTATGTCGTGGCGCACTTC CATTATGTGATGAGCCTCGGCGCGGTCTTCGGCATCTTCGCAGGGATCTACTTCTGGATCGGCAAGATGT CGGGCCGGCAATATCCGGAATGGGCCGGGAAGCTGCATTTCTGGATGATGTTCGTGGGCGCGAACCTCAC CTTCTTCCCGCAGCACTTCCTCGGCCGCCAGGGCATGCCGCGGCGCTACATCGACTATCCCGAGGCCTTC GCCACCTGGAACTTCGTCTCGTCGCTGGGCGCCTTCCTGTCCTTCGCCTCGTTCCTCTTCTTCCTCGGCG TGATCTTCTACACGCTGACGCGCGGGGCGCGGGTGACGGCGAACAACTACTGGAACGAACATGCCGACAC GCTGGAGTGGACGCTGACCTCTCCGCCGCCGGAGCATACGTTCGAGCAGCTTCCCAAGCGGGAAGACTGG GAACGCGCGCCCGCCCACTGA ``` Zaporedje, ki kodira protein 2: ``` ATGGGGCTGGCCTGGGGACTCGGTGTCCTGCTCCTGTTGCATGCCTGCGGCTCCAACCGCATTCCAGAGT CTGGGGGAGACAACAGTGTGTTTGACATCTTTGAACTCACCGGAGCTGCCCGCAAGCGGTCTGGGCGCCG ACTGGTGAAGGGCCCTGACCCTTCTAGCCCAGCTTTCCGCATCGAGGATGCCAACCTGATCCCCCCTGTG CCTGACAAGAAGTTCCAAGACCTAGTGGATGCTGTGCGGGCGGAGAAAGGTTTCCTCCTCCTGGCCTCCC TGAGGCAAATGAAGAAGACCCGGGGTACCCTGCTGGCTGTGGAGCGGAAAGACCACTCTGGCCAGGTCTT CAGCGTGATCTCCAATGGCAAGGCGGGCACCCTGGACCTGAGCCTGACCGTGCAGGGGAAGCAGCATGTG GTGTCGGTGGAAGAAGCACTCCTGGCGACTGGCCAGTGGAAGAGCATCACCCTGTTTGTGCAGGAGGACA GGGCCCAGCTGTACATCGACTGTGAGAAGATGGAGAATGCGGAGCTGGATGTCCCCATCCAGAGCATCTT CACCAGGGACCTGGCCAGCATCGCCAGGCTCCGCATTGCCAAAGGAGGTGTCAACGACAATTTCCAGGGG GTGCTGCAGAATGTAAGGTTTGTCTTTGGAACCACACCAGAAGACATCCTCAGGAACAAAGGCTGCTCCA GCTCTACCAGTGTCTTTGTCACCCTTGACAACAACGTGGTGAATGGGTCCAGCCCTGCCATCCGCACCGA CTACATTGGCCACAAGACAAAGGACCTGCAAGCCATCTGTGGCATCTCATGTGACGAGCTGTCCAGCATG GTCCTGGAGCTCAGGGGTCTACGCACCATCGTGACCACGCTGCAGGACAGTATCCGCAAAGTGACCGAAG AGAACAAAGAGCTGGCCAACGAGCTGAGGAGGCCCCCACTCTGCTACCACAACGGAGTGCAGTACAGGAC TGGCGACGAGTGGACGGTGGACAGCTGCACTGAGTGTCGCTGCCAGAACTCAGTTACCATCTGCAAAAAA GTGTCCTGTCCCATCATGCCCTGCTCCAATGCCACAGTTCCGGATGGAGAATGCTGCCCACGGTGCTGGC CCAGCGACTCTGCAGACGATGGCTGGTCCCCGTGGTCTGAGTGGACCTCTTGCTCTGTGACCTGTGGCAA TGGAATCCAGCAGCGTGGCCGCTCCTGCGACAGCCTCAACAACAGATGCGAGGGCTCCTCTGTGCAGACG CGGACCTGCCACATCCAGGAGTGTGACAAGAGATTTAAACAGGATGGCGGCTGGAGCCACTGGTCCCCAT GGTCATCTTGCTCCGTAACATGTGGAGACGGTGTGATCACAAGGATCCGGCTCTGCAACTCCCCCAGCCC CCAGATGAATGGGAAGCCATGTGAGGGCAAAGCCCGGGAGACCAAAGCCTGCCAGAAAGACTCCTGCCCC ATCAATGGAGGCTGGGGACCTTGGTCACCATGGGACATCTGTTCTGTCACCTGTGGAGGAGGGGTACAGA AACGTAGCCGGCTCTGCAACAACCCCAAACCCCAGTTTGGAGGCAAGGACTGCGTTGGTGATGTGACAGA AAACCAGATCTGCAACAAGCAGGACTGTCCCATTGACGGATGCCTGTCCAATCCCTGCTTTGCTGGTGTC CAGTGTACCAGCTACCCTGATGGCAGCTGGAAGTGTGGTGCCTGTCCCCCAGGCTATAGTGGAGATGGAG TCGAGTGCAAAGACGTTGATGAGTGCAAAGAAGTCCCTGATGCCTGCTTCAACCACAATGGAGAGCACAG GTGTGAGAACACAGACCCCGGCTACAACTGCCTGCCCTGCCCACCGCGCTTCACTGGCTCGCAGCCCTTT GGCCGGGGCGTGGAACATGCCACCGCCAACAAGCAGGTATGCAAGCCCCGAAACCCCTGCACAGACGGGA CACACGACTGCAACAAGAACGCCAAGTGCAACTACCTGGGCCACTACAGCGACCCCATGTACCGCTGCGA GTGCAAGCCTGGCTACGCCGGCAACGGCATCATCTGCGGGGAGGACACAGACCTGGACGGCTGGCCCAAT GAGGACCTGCTGTGCGTGGCCAACGCAACTTACCACTGCAGAAAGGATAATTGCCCCAACCTTCCCAACT CAGGGCAGGAAGACTATGACAAGGATGGAATCGGCGATGCCTGCGATGATGACGATGACAATGATAAGAT TCCAGATGACAGGGACAACTGTCCATTCCATTACAACCCAGCCCAGTACGACTATGACAGAGATGACGTG GGAGACCGCTGTGACAACTGCCCCTACAACCACAACCCAGACCAGGCTGACACAGATAACAATGGGGAAG GAGACGCCTGTGCAGCTGACATTGATGGGGACAGTATCCTCAATGAACGGGACAACTGCCAGTATGTCTA CAATGTGGACCAGAAAGACACTGACATGGACGGGGTTGGTGATCAGTGTGACAACTGCCCCCTGGAACAC AATCCAGACCAGCTCGACTCTGACTCGGACCGCATTGGAGACACCTGTGACAACAATCAGGATATTGATG AAGACGGCCACCAGAACAATCTGGACAACTGTCCCTACGTGCCCAACGCCAACCAGGCTGACCATGACAA GGATGGCAAAGGCGATGCCTGTGACCATGATGACGACAATGATGGCATTCCTGATGACCGGGACAACTGC AGGCTGGTGCCCAATCCTGACCAGAAGGACTCTGATGGTGATGGTCGAGGTGATGCTTGCAAAGATGATT TTGACCAGGACAAGGTGCCAGACATTGATGACATCTGTCCCGAAAATGTTGATATCAGTGAGACTGATTT CCGCCGATTCCAGATGATTCCTCTAGATCCCAAAGGGACATCCCAGAATGACCCTAACTGGGTTGTACGC CATCAGGGTAAAGAACTCGTCCAGACTGTCAACTGTGACCCTGGACTTGCTGTAGGTTATGACGAATTTA ACGCCGTGGACTTCAGTGGCACCTTCTTCATCAACACCGAGAGGGATGACGACTATGCCGGCTTTGTGTT TGGCTACCAGTCCAGCAGCCGCTTCTATGTTGTGATGTGGAAGCAAGTCACTCAGTCCTACTGGGACACC AACCCCACGAGGGCTCAGGGGTACTCTGGACTTTCCGTGAAGGTTGTAAACTCCACCACGGGGCCTGGCG AGCACCTGCGGAATGCCCTGTGGCACACAGGAAACACCTCTGGCCAGGTGCGCACACTGTGGCATGACCC TCGTCACATTGGCTGGAAAGATTTCACTGCCTACAGATGGCATCTGAGCCACAGGCCAAAGACAGGTTTC ATCAGAGTGGTAATGTATGAAGGGAAGAAAATCATGGCTGACTCAGGACCCATCTATGACAAAACCTATG CTGGTGGGAGGCTAGGCTTGTTCGTCTTCTCTCAAGAAATGGTGTTCTTCTCCGACCTGAAATATGAATG CAGAGACTCCTAA ``` ### **ORGANIZMI V FASTA FORMATU** 1. Katerima organizmoma pripadata zaporedji? 2. Kje v celici najdemo protein 1 in kakšno maso ima (Da)? 3. Kako dolg je signalni peptid zaporedja 2? 4. Kakšne posttranslacijske modifikacije so prisotne pri proteinu 2? 5. Poišči homolog proteina 1 v organizmu 2. * Kakšen je max score prvega zadetka? * Zapiši Uniprot kodo homologa. * Zapiši na katerih mestih v zapisu proteina je transmembranska regija 6. 6. Koliko znanstvenih člankov, ki v naslovu vsebujejo znanstveno ime organizma 2, je bilo objavljenih med letoma 2020 in 2022 (vključno s tema dvema letoma)? ### **OD REZULTATOV DO NOVIH POVEZAV** Vaš sodelavec je še vedno na bolniški. Ker ste pomagali že pri identifikaciji vzorcev, vas je vodja raziskovalne skupine zadolžil še za izvedbo nadaljnjih analiz. Preiskujete človeški spondin 1. Spondin 1 je adhezijski protein, ki usmerja in pomaga pri rasti aksonov (*pazi! Iskati je potrebno Spondin-1*). 1. Napiši aminokislinski ostanek, ki se pojavlja v vezavnem mestu spondina 1. * Kateri ion se veže na to mesto? 2. Je ta protein podoben kateremu od zgornjih proteinov? Če ja, koliko mest je identičnih? 3. V katerem ekspresijskem sistemu je bila izražena struktura spondina 1 z resolucijo 2.00 Å? 4. Kakšna je izoelektrična točka spondina 1?